棘球蚴基因工程疫苗免疫绵羊的保护效果观察

Effectiveness of Genetic Engineering Vaccine against Sheep Echinococcus Infection

绵羊棘球蚴病是由棘球绦虫的幼虫感染绵羊所引起一种人兽共患病。该试验在新疆伊犁垦区用棘球蚴基因工程疫苗对8380只6～10月龄绵羊进行了两次免疫;免疫后用间接ELISA方法对不同时期采集的614份血清进行了抗体水平的动态监测;免疫后68周分别对随机挑选的30只免疫组羊和30只对照组羊进行了剖解检查。检测发现,免疫前抗体阳性率为0,首免第4周抗体阳性率达55.56%,随后进行二免,首免第6周～第22周血清样品抗体阳性率均在97%以上。剖解检查发现,免疫组羊细粒棘球蚴包囊感染率为3.33%,对照组感染率达46.00%。试验结果表明,该疫苗安全有效,对绵羊的棘球蚴感染具有很好的免疫保护效果。

Ovine Eehinoeoeeosis is one of zoonosis which caused genetic engineering vaccine was inoculated to 8380 sheep of by Echinococcus larvae. Echinococcus 6-10 month-old two times in Yili reclamation region of Xijiang, and antibody dynamics of 614 serum samples collected in different time were deteeted with indireet-ELISA. After 68 weeks vaeeination, 30 sheep from vaeeinated group and another 30 from control selected randomly were inspected after anatomy. Result shows that the rate of anti-Eg95 antibody is 0 before inoeulation and reached to 55.56% after 4th week of first inoeulation before booster immunization, the positive rate was more than 97% after 6th-22th week after inoculation; the infeetive rata of eehinoeoceus eyst was 3.33% in vaeeinated sheep and 46% in control. The trial shows that Eehinoeoeeus genetie engineering vaccine was safe and effeetive for proteetion against sheep Eehinoeoecus granulosus infeetion.