结肠癌VEGF、TGF-β_1、bcl-2表达与细胞增殖和血管形成的关系

Relationship between expressions of VEGF,TGF-β_1, Bcl-2 and colon carcinoma cell proliferation and angiogenesis

目的研究血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)和bcl-2在结肠癌组织中的表达及其与细胞增殖和肿瘤血管形成的关系。方法采用免疫组化方法检测42例结肠癌患者手术切除后石蜡包埋标本中VEGF、TGF-β1、bcl-2的表达,并根据血管内皮计数判定微血管密度(MVD)。结果结肠癌组织中VEGF、TGF-β1与bcl-2表达主要见于细胞胞浆内,较正常结肠组织表达明显增高。VEGF,bcl-2阳性的结肠癌组织MVD值明显高于VEGF,bcl-2阴性组织中MVD值(P<0.01);TGF-β1阳性癌组织与TGF-β1阴性癌组织MVD值无明显差异性。结论结肠癌组织中VEGF、TGF-β1促进肿瘤细胞增殖和血管的形成,bcl-2抑制肿瘤细胞凋亡,VEGF和TGF-β1与bcl-2无明显相关性。

Objective To investigate the expression of vascular endothelial growth factor （VEGF）,transforming growth factor-betal （TGF-β1） and bcl-2 in colon carcinoma and to evaluate the relationship between expression of VEGF ,TGF-β1,bcl-2 and cellular proliferation activity and angiogenesis. Methods The expression of VEGF, TGF-β1, bcl-2, in the postoperative paraffin section of 42 cases with colon cancer was detected by immunohistochemistry and according to vascular endotheliocyte count, the microvessel density was judged. Results The experessions of VEGF, TGF-β1 and Bcl-2 of colon cancer tissue were seen mainly in the intra-cytoplasma and it was higher than that normal colon tissues. The MVD value of colon cancer tissues of VEGF and Bcl-2 positve was significantly higher than that MVD value of VEGF and Bcl-2 negative（P 〈 0.01）. The MVD value was no obvious difference betweent the TGF-β1 positive and negative cancer tissue. Conclusion TGF-β1 of colon cancer tissue can promote proliferation of tumor cells and angiogenesis. Th Bcl-2 can inbibite anoptosis of tumor cells.