人牙髓血管内皮细胞骨形成蛋白-3基因表达的研究

The expressing of BMP3 mRNA in vascular endothelial cells of human pulpal tissue

目的：研究ＢＭＰ３ｍＲＮＡ在人牙髓血管内皮细胞中的表达。方法：利用地高辛甙元标记的ＢＭＰ３ｃＤＮＡ探针进行原位分子杂交。结果：正常牙髓血管内皮细胞有ＢＭＰ３ｍＲＮＡ阳性杂交信号出现。该信号在龋坏或炎症状态下增强，并在血管周围的未分化间充质细胞中同时出现，但血管瘤组织内血管内皮细胞未发现该阳性信号。结论：人牙髓的血管内皮细胞能合成分泌ＢＭＰ３，可能参与了牙髓的自身稳定和修复过程；ＢＭＰ３ｍＲＮＡ在机体内的分布与组织细胞的胚胎起源和分化状态相关。

Aim: To study the expression of BMP3 mRNA in vascular endothelial cells of human pulpal tissue. Methods: In situ hybridization was used with a digoxigenin-labelled BMP3 cDNA prode. Results: The expression of BMP3 mRNA was observed in human pulpal vascular endothelial cells and also in mensenchymal cells. The expression level was increased in caries and inflammatory pulp. Conclusions: The results indicated that the pulpal vascular endothelial cells could synthesize and secret BMP3, which may be involved in the process of pulp repair; the distribution pattern of BMP3 might be related to the embrologic development.