狗牙髓腔中植入纤维粘连蛋白复合羟磷灰石的实验研究

The response of ectomesenchymal cells of dog dental pulp to implantion of hydroxyapatite paste supplemented with bovine plasma fibronectin

目的：将纤维粘连蛋白（ＦＮ）液与羟基磷灰石（ＨＡＰ）复合并植入狗牙髓腔中，以观察ＦＮ是否改善ＨＡＰ的作用效果。方法：在３只狗４２个牙的唇侧制洞，分别用６００μｇ／ｍｌＦＮ－ＨＡＰ糊剂和生理盐水－ＨＡＰ糊剂植入牙髓腔中，进行组织病理学观察。结果：术后３周，在ＦＮ－ＨＡＰ颗粒周围出现少许骨样基质沉积；术后５周，实验组颗粒周边骨样基质沉积较对照组多。结论：６００μｇ／ｍｌＦＮ液促进狗牙髓组织在ＨＡＰ颗粒周边沉积基质。

Aim: To observe the effects of fibronectin (FN) -hydroxyapatite(HAP) paste on the dog exposed pulp. Methods: 42 teeth of 3 dogs were exposed via buccal class Ⅴ cavities , FN(600μg/ml)-HAP pastes or water-HAP pastes were implanted into the exposed pulps, then sectioned and stained with HE. Results: Three weeks postoperatively, there were some osteomatrix around FN-HAP paste ,five weeks postoperatively, there were stronger new osteomatrix formation around the FN-HAP paste compared with that around HAP paste. Conclusions: FN(600μg/ml) promotes the dog pulp tissues to form osteomatrix around HAP. 