等梯度-HPLC同时检测葡萄酒中5种食品添加剂的研究

Simultaneous determination of five kinds of food additives in wine by isocratic elution HPLC

考察了流动相中乙酸铵与甲醇的配比及流速对苋菜红、糖精钠、苯甲酸、山梨酸、胭脂红分离效果的影响，确定5种添加剂最佳的分析条件，建立了等梯度-HPLC法同时测定葡萄酒中5种添加剂的方法。流动相为0．02M乙酸铵-甲醇（70：30，V/V），等度洗脱，以Diamonsil C18（4．6mmi．d．×150mm，5μm）色谱柱分离，检测波长为λ=230nm。方法对5种食品添加剂有良好的分离效果，在0．80—20．0mg·L^-1浓度范围内呈现良好的线性范围，方法的回收率为96％-103％，精密度为1．7％-2．5％。5种食品添加剂的检测限分别为0．012、0．013、0．010、0．007、0．024mg·L^-1。

The method studied effect of liquid components, flow rate on separation of five kinds of food additives, the best separation parameters was established, the mobile phase was 0.02M CH3 COONH4 - CH3 OH（ 70： 30, V/V）, isocratic eluting ,separation with Diamonsil C18 （4.6 mm i.d. × 150mm,5μm）, detector wavelength was 230nm. The separation effect of method was good, linear range was 0. 80 -20.0mg · L^-1 ,results of recovery test was 96% - 103% ,the RSD was 1.7% -2.5%. The detection limits were 0. 012,0. 013,0. 010,0. 007,0. 024mg · L^-1.