一株产纤溶酶菌株BS-26的分离和鉴定

Isolation and identification of the fibrinolytic enzyme producing strain BS-26

目的对纤溶酶产生菌株进行筛选和鉴定。方法采用LB-纤维蛋白平板初筛和摇瓶发酵复筛的方法进行筛选;通过对菌株形态和生理生化特征以及16S rDNA进行鉴定。结果从土壤中分离得到5株纤溶酶高产菌株,其中菌株BS-26活性最高。其形态和生理生化特征与枯草芽孢杆菌(Bacillus subtilis)很相近。将所测得的16SrDNA序列用BLAST软件与GenBank数据库进行相似性分析,并用Neighbor-Joining法构建系统发育树。BS-26菌株与Bacillus subtilis的相似性达到99.63%。结论BS-26菌株鉴定为枯草芽孢杆菌Bacillus subtilis。

Purpose To isolate and to identify strains producing fibrinolytic enzyme. Methods Strains producing fibrinolytic enzyme were isolated by LB-fibrin plate at first and secondly by shaking flask fermentation. Morphological, physiological and biochemical characteristics and 16S rDNA were used to identify strain.Results Five strains with high fibrinolytic activity were screened from soil and the results indicated that the BS- 26 strain had the highest fibrinolytic activity, and its characters were very similar to Bacillus subtilis. A phylogenetic tree was constructed by comparing with the validly published 16S rDNA sequences of the related strains from GenBank using the Neighbor-Joining method. The 16S rDNA sequence of strain BS-26 shared 99.63 % similarity and probability with Bacillus subtilis. Conclusion BS-26 strain was identified as Bacillus subtilis.