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Review of the Methods for Developing SSR Molecular Markers

Review of the Methods for Developing SSR Molecular Markers
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摘要 Microsatellite marker (or Simple Sequence Repeate, SSR) is a marker technology based on DNA molecular length poly morphism. It is also one of the most commonly used molecular markers. Traditional SSR marker development methods are relatively time-consuming and mostly relying on the known genome sequence information while recently developed methods of SSR marker based on RAPD, ISSR-PCR SSR, the use of hybrid options, sequence tag SSR library access and screening EST-SSR have been widely used. This paper gave an overview of the methods mentioned above for the development of SSR markers. Microsatellite marker (or Simple Sequence Repeate, SSR) is a marker technology based on DNA molecular length poly morphism. It is also one of the most commonly used molecular markers. Traditional SSR marker development methods are relatively time-consuming and mostly relying on the known genome sequence information while recently developed methods of SSR marker based on RAPD, ISSR-PCR SSR, the use of hybrid options, sequence tag SSR library access and screening EST-SSR have been widely used. This paper gave an overview of the methods mentioned above for the development of SSR markers.
出处 《Journal of Northeast Agricultural University(English Edition)》 CAS 2008年第1期62-66,共5页 东北农业大学学报(英文版)
基金 National 863 Program(2006AA10Z1F1)
关键词 SSR marker development method EST-SSR SSR marker, development method, EST-SSR
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参考文献16

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