摘要
目的研究阿魏酸钠(sF)对谷氨酸单钠(MSG)诱导的成年小鼠神经兴奋性毒性的保护作用。方法60只昆明小鼠采用数字随机表法分为对照组、SF组、MSG组、MSG+SF组[根据SF剂量不同,又分为20、40、80mg/(kg·d)三个亚组],每组各10只。MSG组小鼠灌胃MSG[2.0g/(kg·d)],MSG+SF组MSG[2.0/(kg·d)]灌胃的同时腹腔注射SF[20、40、80mg/(kg·d)],SF组腹腔注射SF[40mg/(kg·d)1,每日1次,连续给药10d;对照组灌胃和腹腔注射同量生理盐水。通过小鼠的行为学实验和脑海马组织病理检查.分析MSG所引起的功能和形态学上的变化以及SF的保护作用。结果末次给药后第6天MSG组Y.迷宫正确反应次数比例(13.80/20)显著少于对照组(16.42/20),差异有统计学意义(P〈0.01);MSG[2.0g/(k·d)】+SF[(40mg/(kg·d)]维Y-迷宫正确反应次数比例(16.30/20)与对照组差异无统计学意义胗0.05)。MSG组小鼠海马细胞水肿、神经元变性坏死和组织增生;MSG[2.0g/(kg·d)】+SF【(40mg/(kg·d)]组海马未发现明显的组织病理学改变。结论SF能部分抵消MSG诱导的成年小鼠行为障碍和脑海马病理组织损伤;SF对MSG诱导的成年小鼠神经兴奋性毒性存在保护作用。
Objective To investigate a possible protective effect of sodium ferulate (SF) on monosodium glutamate (MSG)-induced neurotoxicity in adult mice. Methods Sixty mice were randomly divided into control, SF, MSG, and MSG + SF [ 20, 40, 80 mg/(kg.d)] groups, n=10. The animals in MSG group received intragastric (ig) administration of MSG (2.0 g/(kg, d)], the animals in the MSG+SF groups received simultaneously ig administration of MSG [2.0 g/(kg·d)] and intraperitoneal (ip) administration of SF [ 20, 40, 80 mg/(kg· d)], the animals in SF group received ip administration of SF [40 mg/ (kg·d)], and the animals in control group received ig and ip administration of normal saline, respectively, once-daily for 10 d. On day 1 after the last ig administration of MSG or (and) SF the behavioural tests (test of Y-maze discrimination learning and open field test) were performed, and on day 4 after the treatment of MSG or (and) SF the histopathology of the animal brains was studied to analyze the MSG-induced functional and morphological changes and the possible protective effect of SF. Results The correct responses of Y-maze test on day 6 after the last administration of MSG and / or SF in MSG-treated group (13.83/20) were significantly less than those in control (16.42/20)(P〈0.01), and those in MSG[2.0 g/(kg ·d)]+SF[40 mg/(kg .d)]-treated mice (16.30/20) were close to those in control (P〉0.05). Examination of histopathology displayed MSG-treated hippocampal lesions characterized by intracellular edema, degeneration and necrosis of neurons, and hyperplasia, and the hippocampal lesion did not appear in the MSG[2.0 g/(kg.d)]+SF[40 mg/(kg.d)]-treated mice. Conclusions SF partially countered the behavior disorders and hippocampal lesions induced by MSG; therefor, SF has a potent neuroprotection against MSG-induced neurotoxicity in adult mice.
出处
《中华神经医学杂志》
CAS
CSCD
2008年第6期596-599,606,共5页
Chinese Journal of Neuromedicine
关键词
兴奋性氨基酸
神经毒性
神经保护
阿魏酸
Excitatory amino acids
Neurotoxicity
Neuroprotection
Ferulic acid