去卵巢大鼠体质量和骨密度的相关性分析

Correlation between body mass and bone mineral density in ovariectomized rats

目的:探讨去卵巢大鼠体质量与骨密度的相关性,并观察淫羊藿、补骨脂等补肾中药对其作用的影响。方法:实验于2006-09/2007-03在广州中医药大学动物中心和广州中医药大学附属骨伤科完成。①造模:选择雌性未孕的SD大鼠68只,随机分为手术组46只,空白组22只,手术组行双侧卵巢切除,空白组不切除卵巢。术后3个月,从两组中各随机选取10只大鼠,测量体质量和骨密度。②治疗:造模成功后,将手术组剩下36只大鼠随机分为手术模型组、雌激素组、中药组。中药组给予由淫羊藿、补骨脂、丹参、黄芪等10味中药组成的补肾中药4.8g/kg灌胃,1次/d;雌激素组给予尼尔雌醇1mg/kg灌胃,1次/周;另2组给予蒸馏水按10mL/kg灌胃,1次/d。连续治疗3个月。③观察指标:各组大鼠造模前后及治疗后体质量,并用双能X射线骨密度测量仪测量活体全身骨密度、腰椎骨密度、右股骨骨密度和体外右股骨骨密度。结果:①造模后手术组的骨密度低于空白组(P<0.05),体质量明显高于空白组(P<0.05);手术组的骨密度和体质量存在负相关关系(r=-0.854,P<0.05)。②治疗后中药组的活体全身骨密度、腰椎骨密度、活体右股骨骨密度和体外右股骨骨密度高于手术模型组,而体质量低于手术模型组(P<0.05)。③活体和体外右股骨骨密度不存在相关性(r=0.089,P>0.05)。结论:①体质量和骨质疏松的发生有相关性,而补肾中药对提高骨密度有明显的作用。②活体和体外骨密度不存在相关关系,这可能同双能X射线的局限性有关。

AIM： To investigate the correlation between body mass and bone mineral density （BMD） in ovariectomy induced osteoporotic rats, and observe the effects of traditional Chinese herbs of tonifying kidney （epimedium, psoraleae） on rats. METHODS： The experiment was performed in experimental animal center and affiliated Orthopaedic Hospital of Guangzhou University of Chinese Medicine from September 2006 to March 2007. Sixty-eight female non-pregnant SD rats were randomly divided into operation group （n=46） and sham operation group （n=22）. The operation group was ovariectomized, while the sham operation was not. After 3 months, 10 rats each were randomly selected from the two groups to measure the BMD and body mass, respectively. After establishing model, the 36 rats in the OVX group were subdivided into 3 groups： ovariectomized （OVX） group, estrogen supplemented and traditional Chinese herbs group. The rats in traditional Chinese herbs were intragastrically administrated the 4,8 g/kg herbs of tonifying kidney （Epimedium, psoraleae, danshen root, radix astragali and so on） once dally; the rats in estrogen supplemented were i.g. administrated nilestriol, 1 mg/kg, once a week： the others were given distilled water, 10 mL/kg once dally. After 3 months, the BMD of the total rats, lumbar, living right femur and free right femur were measured by dual energy X-ray absorptiometry （DEX2A）, and the body mass of rats was also measured before and after modeling and after treatment. RESULTS： Sixty-four rats were all included in the final analysis. Compared with the sham operation group, the BMD of OVX group was obviously decreased （P 〈 0.05）, and the body mass was increased （P 〈 0.05）. There was a negative correlation between the BMD and body mass in OVX group （ r=-0.854, P 〈 0.05）. After treatment, the BMD of the total rats, the lumbar, living right femur and free right femur were significantly increased, but the body mass decreased in the rats of traditional Chinese herbs compared with OVX group （P 〈 0.05）. There was no correlation in the BMD between the living right femur and free right femur （r=-0.089, P 〉 0.05）. CONCLUSION： Body mass is correlated with the incidence of osteoporosis. Traditional Chinese herbs of tonifying kidney can enhance BMD. There is no correlation in the BMD between the living right femur and free right femur, which may be associated with the limitations of dual energy X-ray absorptiometry.