番石榴叶中皂苷和挥发油抗轮状病毒作用研究

Anti-rotavirus Effects of the Saponin and Volatile Oil from Psidium Guajava Leaves

目的利用四氮唑(MTT)法检测番石榴叶中皂苷、挥发油的抗轮状病毒作用。方法提取番石榴叶中皂苷和挥发油两种有效成分,将其作用于MA104细胞,MTT法检测细胞存活率,求出其半数细胞毒性浓度(TC50)后,给予<TC50不同浓度的有效成分对轮状病毒感染细胞进行干预,检测其对病毒感染细胞存活的影响,以确定两种有效成分体外抗轮状病毒的作用。结果皂苷和挥发油均有预防轮状病毒感染的作用,其用于预防时病毒最高抑制率分别为62.12%和60.04%,治疗指数(TI)分别1.69和1.50。同时,皂苷和挥发油对MA104细胞感染病毒后具有治疗作用,两者用于治疗轮状病毒感染时最高抑制率分别为78.37%和86.55%,TI分别为3.30和3.59。,随着药物浓度的增加,治疗和预防时病毒抑制率明显升高,呈量效关系。结论番石榴叶中皂苷、挥发油具有良好的体外抗轮状病毒的作用。

Objective To evaluate anti-rotavirus effects of the saponin and volatile oil from psidium guajava leaves by MTT assay. Methods The saponin and volatile oil were extracted from psidium guajava leaves, and added into MA104 cells. Afterwards, the cell viability was detected by MTT assay. Calculating TC50, different dose of the two components were added into rotavirus infected cells to determine their anti-rotavirus effects in vitro. Results It was found that the two components inhibited the infection of MA104 cells by rotavirus, the maximum suppression ratio were 62.12% and 60.04% , respectively for prophylactic use, the therapeutic index TI were 1.69 and 1.50. Meanwhile, the two components have therapeutical effects on rotavirus infected cells, the maximum inhibition rate were 78.37% and 86.55% , and the TI were 3.30 and 3.59, respectively. The anti-virus effects showed a dose related manner. Conclusion The saponin and volatile oil from psidium guajava leaves have strong anti-rotavirus effects in vitro.