气态甲醛对小鼠外周血淋巴细胞和肝细胞微核率的影响

Effect of micronucleus rate in peripheral lymphocytes and liver cells of mice exposed to gaseous formaldehyde

目的研究气态甲醛对细胞遗传物质的毒性。方法选用SPF级昆明种纯系雄性小鼠作为研究对象,用浓度为0.5、1.0和3.0mg/m3的气态甲醛对小鼠进行连续动态染毒72h,染毒结束后观察外周血淋巴细胞和肝细胞微核形成率。结果气态甲醛能诱导外周血淋巴细胞微核率和肝细胞微核率增加,染毒组与对照组比均有显著性差异。结论甲醛对小鼠外周血淋巴细胞和肝细胞的染色体有明显的损伤作用。

Objective To explore the potential damage of gaseous formaldehyde on genetic material of cells. Methods SPF-class Kun -Ming male mice were used in the experiment. The mice were exposed to gaseous formaldehyde in different concentrations ：0. 5 mg/m3 .1.0 mg/m3 and 3.0 mg/m3 ,the agent was administrated by continuous dynamic inhalation for 72 hours. After the exposure to formaldehyde, micronucleus rate in peripheral lymphocytes and liver cells was measured. Results Gaseous formaldehyde could induce the increase of micronucleus rate in peripheral lymphocytes and liver cells. It indicates that there is significant difference between toxicity group and control group. Conclusion Gaseous formaldehyde has significant damage effect on chromsome in peripheral lymphocytes and liver cells.