气态甲醛暴露对神经生长因子表达影响的体内实验

Nerve growth factor expression in mice and rats induced by gaseous formaldehyde exposure in vivo

为了探讨气态甲醛对小鼠组织中NGF表达的诱导作用,以小鼠肺和脑作为实验材料,应用RT-PCR方法检测甲醛染毒后小鼠肺和脑中NGF-mRNA的表达量.结果显示,小鼠脑中NGF表达量随着染毒时间的增加而增加;而肺中NGF在甲醛染毒1d后就达到最大,但随着时间的增加NGF表达量反而下降;故NGF在小鼠这2种组织中呈现不同的表达效应.为了进一步研究甲醛、NGF和哮喘之间的联系,构建了大鼠的哮喘模型,应用RT-PCR方法检测哮喘模型鼠肺中NGF的表达趋势.结果显示,在大鼠哮喘模型中,低浓度甲醛组(1.0mg·m-3)NGF表达量显著上调(p<0.01),而高浓度甲醛组(3.0mg·m-3)NGF表达量反而下降,仅略高于空白对照组(p>0.05).

Expression of Nerve Growth Factor （NGF） mRNA in lung and brain tissues of mice, as induced by gaseous formaldehyde was explored. RT-PCR was applied to assess NGF mRNA expression levels in the two tissues after formaldehyde inhalation in vivo. The results showed that NGF mRNA expression levels in mice brain increased with formaldehyde inhalation time. Compared with expression in the brain, there were higher expression levels of NGF mRNA in mice lung after 1 day, but the expression level dropped as the formaldehyde inhalation time increased. So there were different expression effects of NGF mRNA in the two tissues. A rat asthma model was built to investigate the relationships among formaldehyde, NGF and asthma. RT-PCR was used to assess NGF mRNA expression levels in rat lungs. In the rat asthma model, NGF mRNA expression levels of low formaldehyde-induced groups （1.0 mg·m^-3） were up-regulated significantly （p〈0.01）, but the levels of high formaldehyde-induced groups （3.0 mg·m^-3） dropped little compared with the low formaldehyde induced groups, and were just higher than the control groups （p〉0.05）.