以自体诱导和无诱导的骨髓基质干细胞复合珊瑚修复犬下颌骨节段缺损的比较研究

Comparative Study in Repair of Canine Segmental Mandibular Defects Using Different Coral-engineered Bone

目的分别采用诱导和无诱导的自体骨髓基质干细胞(Bone marrow stromal cells,BMSCs)复合珊瑚构建组织工程化骨,修复犬下颌骨节段性缺损,比较修复效果。方法体外扩增、成骨诱导或无诱导培养犬BMSCs,分别将第2代细胞复合珊瑚后修复犬自体右侧3cm的下颌骨节段缺损(诱导组n=6,无诱导组n=6)。术后32周,分别通过Micro-CT、大体形态观察和组织学方法检测骨缺损的修复效果。结果32周时,Micro-CT检测示诱导组骨容积率和密度均显著高于对照组(p<0.05);大体观察示诱导组骨愈合良好,无诱导组中的3条犬为骨不连;组织学检测诱导组有较多成熟骨形成,缺损部分均呈骨性愈合,无诱导组中的3只犬有新骨形成,但形态不完整,另3只犬的缺损部分呈纤维性愈合。结论成骨诱导的自体BMSCs复合珊瑚形成的组织工程化骨修复犬下颌骨节段缺损效果优于无诱导组。

Objective To compare the therapeutic effect in repair of canine segmental mandibular defects between two different coral-engineered bone （osteogenic induced bone marrow stromal cells-coral and non-induced BMSCs-coral）. Methods Isolated BMSCs were in vitro expanded and osteogenic or non-osteogenic induced. In 12 canines, a 3 cm segmental mandibular defect at right mandible was created. 6 canine＇s defects were repaired with cell-scaffold constructs made from induced BMSCs and coral; Others were repaired with cell-scaffold constructs made from non-induced BMSCs. The engineered bone was evaluated by Micro-CT, gross and histological examination at 32 weeks post-operation respectively. Results At 32 weeks, Micro-CT revealed significantly higher bone volume and mineral density of induced group than non- induced group （p〈0.05）. Moreover, gross observation demonstrated well bony-union of induced group, while bony-nonunion in 3 canines of non-induced group. Also Histologically, mature bone were commonly observed and there were bony healing of induced group, while fibrous healing occured in non-induced group. Conclusion Canine segmental mandibular defects can be better repaired by osteogenic induced BMSCs-coral engineered bone than non-induced BMSCs-coral engineered bone.