马唐致病菌株Mds0404的生物学特性

The biological characteristics of pathogenic strain Mds0404 of Curvularia sp.to Diditaria sanguinaris

研究了影响马唐生防菌弯孢霉Mds0404菌株营养生长、孢子萌发及孢子形成的因子。结果表明，该菌在PSA培养基上生长最好，在PDA培养基上产孢最多；在10～40℃均可生长，最适生长温度为25～32℃；在20～32℃均可产孢，最适产孢温度为25～28℃；pH为5时生长最快，pH为8时产孢量最大；在以蔗糖为碳源、硝酸钾为氮源的Czapek培养基上生长与产孢均较好；加膜25cm紫光灯12h间隔光照产孢最丰富。分生孢子萌发的温度为10～40℃，最适为25～40℃，失活温度为55℃。黑暗有利于孢子的萌发。16min近距离紫外光照射萌发率仍保持在50％以上。

Factors influenced vegetative growth, conidial germination and sporulation of strain Mds0404 of Curvularia sp., which was a biological control agent for crabgrass （Digitaria sanguinaris ）, had been primarily studied. PSA was the best medium for growth of pathogenic strain and PDA was the best for sporulation. The ranges of temperature for mycelium growth was 10 - 40℃, the optimum was 25 - 32℃. The range of temperature for fungus sporulation was 20 - 32℃, the optimum at 25 - 28℃. The optimum pH for mycelia growth and for the amount of six）ms produced was pH 5 and pH 8, respectively. The fungus grew and spared better in Czapek medium with sugar as carbon source and KNO3 as nitrogen source. The amount of spare produced most when the medium was lighted under purple lamp covered with membrane away from 25 cm at 12 hours interval. The range of temperature for conidial germination was 10 - 40℃, the optimum at 25 - 40℃, the temperature of deactivation was 55 ℃. Darkness was favor of conidial germination. The percentage of spore germination was still over 50% under UV-light irradiation for 16 minutes.