阻断NF-κB/IκB信号通路对HIBD大鼠脑内炎症因子的影响

Effects of regulation of NF-κB/IκB inflammation signal transduction pass on cytokine of HIBD rat model

目的:建立大鼠缺氧缺血性脑损伤(HIBD)模型,将IκB通过腺病毒载体导入体内,探讨其对HIBD大鼠脑内NF-κB活性和TNF-α、IL-1表达的影响。方法:40只大鼠随机分为5组:I组(正常对照组),II组(HIBD损伤1d组),III组(HIBD损伤7d组),IV组(IκB干预1d组),V组(IκB干预7d组)。Western-blot检测脑细胞NF-κB的表达,了解NF-κB的活性,放免法检测TNF-α、IL-1的表达。结果:经中心静脉途径导入腺病毒转载的IκB基因,可降低HIBD大鼠脑内NF-κB活性,IκB干预1d组和7d组与HIBD损伤1d组和HIBD损伤7d组比较,NF-κB活性明显减少(P<0.05);降低脑内TNF-α、IL-1含量,IκB干预1d组和7d组与HIBD损伤1d组和7d组比较,TNF-α、IL-1含量明显减少(P<0.05)。结论:通过中心静脉途径注入腺病毒转载的IκB基因,直接增加脑内IκB的表达,阻断炎症因子TNF-α的合成,机制可能与其抑制了NF-κB的活性有关。

Objective ：To explore the effect of inflammation reaction of HIBD rat model by infusing IκB gene into the rat model with adenovirus vector through the central vein. Methods：Forty rats were randomly divided into five groups：Group Ⅰ （control group） ,Group Ⅱ （HIBD 1d）,Group Ⅲ （HIBD 7d）,Group Ⅳ （Ad IκB therapy ld） ,and Group V （Ad IκB therapy 7d）. The expression of NF--κB in the brain tisse was measured by Western--blot method. The levels of TNF--α and IL--1 in the brain tisse were recorded after injection at day 1 and 7. Results：After infusing IκB gene-- tansported-- adenovirus through central vein, the NF--κB expression in IκB therapy at day 1 and IκB therapy at day 7 was significantly lowered in comparison with that in HIBD ld group and HIBD 7d group, and the cytokine level was significantly reduced （P 〈 0.05） . Conclusion ：It may be a new direction for therapy HIBD by increasing expression of IκB gene by infusing IκB gene-- tansported-- adenovirus through the central vein, and the possible neuroprotection mechanism of HIBD may be related to the activity of NF--κB.