Rb在婴幼儿皮肤血管瘤不同时期的表达及意义

Expression of Rb in infantile cutaneous hemangioma

目的:探讨Rb蛋白在血管瘤发生、发展及退化过程中的表达状况及其意义。方法:采用免疫组织化学方法(SABC法)检测人皮肤血管瘤增生期、退化期及正常皮肤组织中Rb的表达水平,并结合第因子相关抗原的免疫组织化学染色证实表达Rb的细胞是血管内皮细胞。利用计算机图像分析技术测量不同时期血管瘤组织和正常皮肤组织Rb表达的积分光密度和面积。结果:增生期血管瘤内皮细胞Rb表达水平低于退化期,差异有显著性(P<0.05),退化期血管瘤内皮细胞Rb表达水平与正常皮肤组织相比,差异有显著性(P<0.05)。结论:Rb通过抑制血管瘤内皮细胞增殖和血管生成而在血管瘤的退化过程中起重要作用。

Objective： To investigate the expression and significance of Rb in the occurrence, development and regression of infantile hemangiomas. Methods：The expression of Rb was examined in the proliferative stage and catagen of human hemangiomas and normal skin tissues by using immunohistochemical technique. Immunohistochemical technique for factor Ⅷ -- related antigen was used to prove that the cells which expressed Rb were endothelium. Image analysis system was applied to measure the expression level of Rb at different stages of hemangiomas and in normal skin tissues. Results：The expression of Rb was significantly lower in proliferating hemangiomas than that in involuting hemangomas （P ~ 0.05）. Conclusion： Rb might play an important role in the regression of human hemangioma endothelial cells and anti--angiogenesis.