摘要
目的探讨牙周局部注射不同浓度的1,25-二羟基维生素D3[1,25-(OH)2D3]对大鼠正畸牙移动压力侧破骨细胞分化因子表达的影响,以及相关的作用机制。方法96只成年健康雄性Wistar大鼠随机等量分为对照组、A组、B组和C组。在大鼠上颌左侧第一磨牙与上颌两切牙之间安放加力装置,每隔3d注射药物10μL;对照组注射生理盐水,A组注射10-10mol/L的1,25-(OH)2D3,B组注射10-8mol/L的1,25-(OH)2D3,C组注射10-6mol/L的1,25-(OH)2D3;分别于加力后第1、3、7、14天处死各组中6只大鼠。制取标本后,HE染色观察正畸牙移动牙周膜压力侧破骨细胞的数目、形态以及活性变化,同时运用免疫组织化学染色,检测正畸牙压力侧破骨细胞分化因子(ODF)的表达。结果4组大鼠正畸牙移动压力侧破骨细胞的变化趋势一致,但是B组大鼠压力侧ODF表达在第7、14天显著提高。结论10-8mol/L的1,25-(OH)2D3能更有效地促进大鼠正畸牙的移动过程中ODF的表达,促进破骨细胞的活化。
Objective To observe expression of ODF during orthodontic tooth movement with injecting of 1,25- （OH） 2D3 of different concentrations in rats. Methods 96 adult male Wistar rats were randomly divided into four groups： Control group （local injection with sodium chloride）; Group A [ local injection with 10^-10 mol/L 1,25- （OH）2D3 ] ; Group B [local injection with 10^-8 mol/L 1,25-（OH）2D3 ] and Group C [local injection with 10^-6 mol/L 1, 25-（ OH）2D3 ]. An orthodontic appliance was devised to make the maxillary left molar mesially. 1,25-（OH）2D3 was injected every 3 days. The animals were sacrificed at 1,3,7 and 14 days. The population of osteoclasts at compressive side were measured and comparatively analyzed. The expression of ODF was detected with immunohistochemical staining. Results The expression of ODF was significantly changed in a time-dependent manner, ODF expression reached maximum in 7 days, and decreased in 14 days. ODF expression of Group B was increased significantly （P 〈0.05）. Conclusion Using 10^-8 mol/L 1,25-（OH）2D3 during orthodontic tooth movement could increase positive expression of ODF at the compressive side.
出处
《广东牙病防治》
2008年第6期259-262,共4页
Journal of Dental Prevention and Treatment
