刺参黏多糖对人宫颈癌Hela细胞caspase表达影响

EFFECT OF STICHOPUS JAPONICUS SEKENKA ON CASPASE EXPRESSION OF HELA CELLS OF CERVIX NEOPLASMS

目的 探讨刺参黏多糖对人宫颈癌Hela细胞株体外生长、凋亡的影响及可能机制。方法 以0～8.0 g/L刺参黏多糖分别处理Hela细胞24～120 h后,以四甲基偶氮唑蓝比色法（MTT）测定细胞生长活性,透射电镜观察细胞形态,RT-PCR法检测凋亡相关基因caspase-3c、aspase-9 mRNA的表达。结果 刺参黏多糖对Hela细胞的生长具有抑制作用,电镜下可观察到凋亡小体等细胞凋亡的形态学改变。随着药物浓度及作用时间的增加,caspase-3及casepase-9 mRNA表达增加。结论 刺参黏多糖可抑制Hela细胞生长,诱导Hela细胞的凋亡,其机制可能是通过诱导caspase-3及caspase-9 mRNA表达增多实现的。

Objective To study the apoptosis of Hela cells of cervix neoplasms induced by stichopus japonicus sekenka （SJAMP） and its possible mechanism. Methods Hela cells were treated with different doses of SJAMP on different time. The inhibitory effect on cell proliferation was assayed by MTT, the apoptosis observed by electronic microscopy. The expression of caspase-3 and caspase-9 was assayed with reverse transcription-polymerase chain reaction （RT-PCR）. Results SJAMP suppressed the growth of Hela cells obviously in dose-and time-dependent manners. Morphological changes of cell apoptosis, such as karyopyknosis and conglomeratio, were observed electronic microscopically. RT-PCR assay showed the expression of caspase-3 and caspase-9 increased after exposure to different doses of SJAMP on different time. Conclusion SJAMP can inhibit proliferation and induce apoptosis of Hela cells, caspase3 and caspase-9 may have an important rule in the regulation of cell apoptosis.