核糖核酸诱导肝癌细胞株HEPG2凋亡的实验研究

The study of ribonucleic acid inducing the apoptosis of hepatoma cell strain-HEPG2

目的:研究核糖核酸对肝癌细胞株HEPG2凋亡的作用。方法:将肝癌细胞株HEPG2分为对照组、喜树碱(CPT)组和核糖核酸3组。核糖核酸组又分为剂量效应组和时间效应组。采用流式细胞仪对凋亡细胞进行定量检测。结果:随着培养时间延长,核糖核酸组HEPG2细胞凋亡逐渐增加,在诱导后20小时细胞凋亡率达到最高,为38·6%,其浓度以20μl/ml为最佳药物作用浓度。结论:核糖核酸对HEPG2细胞具有促凋亡作用,且具有剂量和时间依赖性。

Objective： To study the effect of ribonucleic acid inducing the apoptosis on hepatoma cell strain- HEPG2. Methods： The hepatoma cell strain-HEPG2 were divided into three groups, control group, camptothecine （CPT） group and ribonucleic acid group. The ribonucleic acid group was divided into dosage effect group and time effect group. The quantition detection of the apoptosis of hepatoma cell strain-HEPG2 was observed with flow cytometer. Results： With the cell cultivation time lasting, the apoptosis of hepatoma cell strain-HEPG2 in ribonucleic acid group was incresing gradually. The apoptosis rate 38.6% was the highest in 20h after inducing by ribonucleic acid. The best concentration was 20μl/ml for HEPG2. Conclusion： The ribonucleic acid has an effect of promoting the apoptosis of HEPG2, and has a time and concentration dependence.