软肝煎对非酒精性脂肪肝大鼠肝纤维化的预防作用

The study on the prevention of nonalcoholic steatohepatitis with fibrosis by Ruangan compound

目的:观察软肝煎对非酒精性脂肪肝大鼠肝纤维化的预防作用,并探讨其作用机制。方法:将38只SD大鼠随机分为4组,除正常组(N)外,其余3组即模型组(M)、软肝煎低剂量组(L)、软肝煎高剂量组(H)大鼠用高脂饲料喂养共16周,制备大鼠非酒精性脂肪肝模型。L组、H组大鼠以软肝煎低、高剂量灌胃,1次/d,共12周;N、M组大鼠以等量生理盐水灌胃。观察各组大鼠肝组织病理变化及TGF-β1阳性表达情况,检测血清PCⅢ、CⅣ、HA、LN水平,并进行比较。结果:M组大鼠肝组织呈脂肪变、炎性细胞浸润、肝细胞坏死、纤维组织明显增生,L、H组大鼠肝组织病理表现较M组明显减轻;M组大鼠TGF-β1阳性表达较N组增高(P<0·05),而H组较M组则降低(P<0·05);M组大鼠各项血清学指标较N组明显升高(P<0·05),而L、H组较M则明显降低(均P<0·05),且此4项指标与肝纤维化存在正相关。结论:软肝煎不仅可以治疗脂肪肝、高脂血症,而且还具有确切地预防非酒精性脂肪肝纤维化作用。

Objective： To study the preventive effect of Ruangan compound in nonalcoholic steatohepatitis with fibrosis. Methods： Thirty-eight SD rats were divided randomly into four groups： control group was fed with normal diet; model group, low dose group and high dose group were fed with a fat-rich diet. After 16 weeks, low dose group and high dose group were perfused with Chinese herbs for 12 weeks. The serum levels of HA, LN, PCⅢ and CⅣ were measured. Liver pathology and positive express degree of cytokine TGF-β1 were observed. Results： Pathology： All rats of model group developed steatosis mixed with inflammatory cell infiltration and necrosis. Central veins peripheral fibrosis and perisinosodial fibrosis also could be observed in model group. Low dose group and high dose group were better than model group. Serum levels： Model group was significantly higher than control group （ P 〈 0. 05 ） . Compared with model group, low dose group and high dose group had significantly lower levels （P 〈 0. 05 ） . Cytokine TGF-β1 ： Compared with control group, model group had significantly higher levels （P 〈 0. 05 ） . High dose group was significantly lower than model group （ P 〈 0. 05 ） . Conclusion： Ruangan Compound not only can therapy nonalcoholic adiposis hepatica, hyperlipoidemia and protect hepatocyts from injury, but also can prevent the emergence of nonalcoholic steatohepatitis with fibrosis.