玻璃体内注射曲安奈德对兔眼的毒性作用

Ocular toxicity of intravitreal injection of triamcinolone acetonide in rabbit eyes

目的观察玻璃体内注射曲安奈德对兔角膜、晶状体、睫状体和视网膜的毒性作用。方法32只灰兔分为四组(n=8),玻璃体内分别注射乳酸林格氏缓冲液(对照组)、4 mg曲安奈德(B组)、1.3 mg曲安奈德(C组)和载体(D组)。于注射前和注射后不同时间点分别进行眼压及明、暗适应视网膜电图检查;于术后1周、1月和3月,每组分别取2眼观察组织学和超微结构的改变。结果与其他各组比较,B组注射后1 d和1、2周的眼压显著上升(P<0.05)。术后1周和1月,B组和D组视网膜电图各峰值较对照组和C组明显下降(P<0.05)。光镜下B组视网膜组织受损;电镜下B组和D组的睫状体、晶状体和视网膜组织均不同程度受损。结论玻璃体内注射曲安奈德和载体后,对兔晶状体、睫状体和视网膜组织可能有一定的毒性作用。

Objective To evaluate the ciliary body and retina in rabbit eyes toxicity of intravitreal injection of triamcinolone acetonide on the cornea, lens, Methods Thirty-two gray rabbits were divided into four groups （n = 8）. The rabbits were intravitreally injected with buffered saline solution （control group）, 4 mg triamcinolone acetonide （group B）, 1.3 mg triamcinolone acetonide （group C ） and vehicle （group D ）. Intraocular pressure, scotopic and photopic electroretinogram examinations were performed before injection and at different time points after injection. Histologic and ultrastructural changes were observed 1 week, 1 month and 3 months after injection. Results Compared with the other groups, the intraocular pressure of group B was significantly increased on day 1, week 1 and week 2 after injection （P 〈 0. 05）. The amplitudes of electroretinogram of group B and D were significantly decreased than those of control group and group C on week 1 and month 1 after injection （ P 〈 0.05）. It was revealed by light microscopy that the retina was impaired in group B. The impairment of lens, ciliary body and retina in group B and D was detected by electron microscopy. Conclusion There may exist toxicity to the lens, ciliary body and retina in rabbit eyes after intravitreal injection of triamcinolone acetonide and vehicle.