实验性碱烧伤角膜新生血管的观察研究被引量：1

Observation of experimental corneal neovascularization induced by alkali burn

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摘要目的利用碱烧伤诱导实验性角膜新生血管(CRNV)的发生,探讨定量分析CRNV和观察CRNV通透性的方法。方法取36只C57BL/6小鼠,随机分为:实验组(n=30):采用浸润l mol/L NaOH的滤纸片接触角膜5 s,诱导碱烧伤CRNV;对照组(n=6):不进行任何处理。于碱烧伤后第4、7、10、14天测量CRNV面积;第3、7、10、16、28天取眼球做切片的HE染色行组织学检查;第10天使用CD31抗体标记CRNV并计数,行荧光血管造影观察CRNV的通透性;每日观察角膜溃疡和前房积血等情况。结果实验性碱烧伤CRNV存在生长和消退的过程。无菌性角膜溃疡和前房积血较为常见,发生率分别为6.7%和10.0%。眼球切片的HE染色可观察到不同时间角膜组织的病理变化。碱烧伤后第10天,CD31抗体免疫组化标记并记数CRNV,荧光血管造影可观察到显著的CRNV渗漏。结论CD31抗体免疫组化标记、记数CRNV及联合CRNV面积测量,是CRNV定量分析较为客观的方法;荧光造影是观察和记录CRNV通透性可行的方法。 Objective To induce the experimental corneal neovascularization （CRNV） by alkali burn, and explore the methods for quantitative analysis of CRNV and observation of permeability of CRNV. Methods Thirty-six C57BL/6 mice were selected and divided into experiment group （ n = 30） and control group （n = 6）. For the experiment group, alkali burn was induced by application of filter paper with lmol/L sodium hydroxide to the cornea for 5 s. For the control group, no intervention was conducted. Areas of CRNV were measured on day 4, 7, 10 and 14 after alkali burn. Histological examinations of cornea were performed with HE staining on day 3, 7, 10, 16 and 28 after alkali burn. On day 10, endothelial Cell marker CD31 was used with immunohistochemical staining for CRNV counting,and fluorescence angiography （FA） was employed to reveal the permeability of CRNV. Corneal ulceration and hyphema were observed everyday. Results CRNV developed after alkali burn, and extincted afterwards. Axenic coneal ulceration and hyphema were frequently observed, with the incidences of 6. 7% and 10.0% , respectively. Histologic changes of corneal tissues at different time points could be observed with HE staining. On day 10, CRNV could be labeled and counted with immunohistochemical staining for CD31 antibody, and the permeability of CRNV could be detected by FA. Conclusion CRNV counting with immunohistochemical staining for CD31 antibody and measurement of area of CRNV are appropriate methods for quantitative analysis of CRNV. FA is an effective method in the detection of permeability of CRNV.

作者左磊许迅樊莹王丰顾青

机构地区上海交通大学第一人民医院分院眼科上海交通大学第一人民医院眼科上海交通大学第一人民医院中心实验室上海交通大学第一人民医院眼科研究所

出处《上海交通大学学报（医学版）》 CAS CSCD 北大核心 2008年第6期656-660,共5页 Journal of Shanghai Jiao tong University:Medical Science

关键词角膜新生血管碱烧伤小鼠荧光血管造影 corneal neovascularization alkali burn mouse fluorescence angiography

分类号 R779.1 [医药卫生—眼科]

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