应用胶原-壳聚糖桥接管引导大鼠坐骨神经再生

Repair of sciatic nerve defects with a collagen protein-chitosan complex conduit in rat

目的:观察胶原-壳聚糖桥接管促进大鼠坐骨神经损伤后再生与修复的作用。方法:用壳聚糖和胶原蛋白按1∶3的比例采用冷冻干燥法制成胶原-壳聚糖复合导管;将20只雄性Wistar大鼠随机分为2组,胶原-壳聚糖导管组和硅胶管组,切断坐骨神经,建立10mm的缺损动物模型;分别用胶原-壳聚糖导管和硅胶管进行桥接。于术后不同时间对2组动物进行大体观察,并于术后14周进行电生理、组织学及逆行示踪检测,比较2组大鼠坐骨神经的再生和功能恢复情况。结果:术后14周2组动物坐骨神经再生和功能恢复情况的各项检测指标显示,胶原-壳聚糖桥接组明显优于硅胶管桥接组。结论:胶原-壳聚糖导管可用来桥接损伤神经,在周围神经缺损修复方面具有良好的应用前景。

Objective： To evaluate the effects of collagen protein-chitosan complex conduits on the regeneration of peripheral never. Methods ： Complex chitosan conduits were made from chitosan and collagen protein by the method of freeze drying in the proportion of 1 to 3. Twenty Wistar rats were divided into collagen protein-chitosan complex conduit group and silicone tube group. Ten mm defects in the sciatic nerves were bridged with the collagen protein-chitosan complex conduit or a silicone tube. At different time after grafting, the regenerated nerves were evaluated by electrophysiological examination, histological staining and retrograde tracing. Results： At 14 weeks after grafting, all results from collagen protein-chitosan complex conduit group were better than that from silicone tube group. Conclusion ： The collagen protein-chitosan complex conduit offers a possible substitute for repairing the defects of the peripheral nerve.