血管内皮生长因子及其受体mRNA在输卵管妊娠蜕膜组织的表达

Messenger RNA expression of vascular endothelial growth factor and its receptors in tubal pregnant decidual tissue

目的:检测血管内皮生长因子(VEGF)及其受体(KDR、Flt-1)在输卵管妊娠蜕膜组织的表达,探讨其在输卵管妊娠中的作用。方法:采用半定量RT-PCR方法,检测输卵管妊娠蜕膜组织中的VEGF、KDR、Flt-1 mRNA的表达,并与正常输卵管黏膜及正常宫内早孕子宫蜕膜组织比较。结果:半定量结果表明,VEGF、KDR、Flt-1 mRNA在输卵管妊娠蜕膜组织中的表达强于正常输卵管组织,但弱于正常宫内早孕蜕膜组织,差异均有显著性。结论:输卵管妊娠时,VEGF及其受体mRNA水平的增高是使滞留在输卵管的胚泡着床于输卵管的重要因素。

Objective： To investigate messenger RNA expression of vascular endothelial growth factor （VEGF） and its receptors in tubal pregnant decidual tissue and to explore their effects on tubal pregnancy. Methods： The mRNA expressions of VEGF, kinaseinsert domain-containing receptor （KDR） and fms-like tyrosine kinase 1 （flt-1） in the tubal pregnant decidual tissue, the normal oviductal tissue and the early pregnant human endometrial decidual tissue were detected and compared with semiquantitative reverse transcription polymerase chain reaction. Results： The mRNA expression of VEGF and its receptors, KDR and flt-1, was significantly higher in the tubal pregnant decidual tissue compared with the normal oviductal tissue, but lower than that in the early pregnant human endometrial decidual tissue. Conclusion： The higher expression of VEGF and its receptors may be a remarkable factor responsible for the implantation in the oviduct.