异丙酚预处理对拟阿尔茨海默病模型大鼠神经行为学和神经原纤维缠结及胶质纤维酸性蛋白的影响

Effects of propofol pretreatment on neurological behavior,neurofibrillary tangles and expression of GFAP in Alzheimer disease-like model rats

目的:观察异丙酚预处理对拟阿尔茨海默病(AD)模型大鼠的神经行为学及海马损伤神经原纤维缠结(NFT)和胶质纤维酸性蛋白(GFAP)表达的影响,分析异丙酚的脑保护作用。方法:大鼠海马CA1区微量注射冈田酸(OA),建立拟AD大鼠模型。异丙酚预处理组大鼠于拟AD模型制作前30min,腹腔注射异丙酚100mg/kg。并以Morris水迷宫实验观察大鼠行为学变化,Bielschowsky染色观察NFT及免疫组织化学方法观察GFAP表达的变化。结果:与对照组大鼠相比,模型组大鼠学习记忆能力显著降低;Bielschowsky染色观察海马CA1区域出现大量NFT的特征性病理变化,GFAP免疫组织化学阳性细胞明显增多。与模型组大鼠相比,异丙酚预处理组大鼠学习记忆能力明显提高;海马CA1区NFT明显减少或消失,GFAP免疫组织化学阳性细胞明显减少。结论:拟AD模型建立前30min异丙酚预处理能改善拟AD模型大鼠学习记忆能力,降低NFT和GFAP的表达,有明显的脑保护作用。

Objective： To study the effects of propofol pretreatment on neurological behavior and the expression of neurofibrillary tangles （NFT） and glial fibrillary acidic protein （GFAP） of hippocampus tissues in Alzheimer disease-like model rats, and to analyze the protective effects of propofol on the brain. Methods ： Okadaic acid （OA） was injected into hippocampal CA1 region of rats in the model group and the pretreatment group to establish Alzheimer disease-like model. Rats in the pretreatment group were peritoneally injected with propofol （100 mg/kg） before mading AD-like model. The learning and memory abilities of rats were assessed through Morris water maze behavioral test. NFT, the characteristic pathological changes of AD, were detected by Bielschowsky stain of hippocampus, and the expressions of GFAP were examined by immunohistochemistry in the hippocampus CA1 region of the rats. Results ： Compared with the control group, the abilities of learning and memory of the rats in the model group were significantly decreased. Many NFTs were observed in the hippocampal CA1 region. The expression of GFAP in the CA1 region increased. Compared with the model rats, the abilities of learning and memory of the rats in the propofol group rats improved. NFT was reduced in the hippocampal CA1 region. The expressions of GFAP in the CA1 region decreased. Conclusion ： Propofol treatment at 30 minutes before OA injection can ameliorate the neurobehavioral score of rats with hippocampus injury, and reduce the level of NFT and GFAP in the brain tis sues of the rats with OA injury, which has significant protective effects on the brain.