茶树菇活性提取物抗氧化和延缓衰老作用的研究

STUDY ON THE ANTIOXIDATIVE AND ANTIAGING EFFECT OF THE BIOACTIVE EXTRACT FROM AGROCYBE AEGERITA

目的探讨茶树菇活性提取物的抗氧化和延缓衰老作用。方法分别用茶树菇活性提取物培养果蝇和灌胃衰老模型小鼠,观察其对果蝇寿命以及小鼠血清SOD活性、肝脂褐素含量、脾指数和脑指数的影响,并进行急性毒理试验。结果20g/L的茶树菇活性提取物能极显著延长雄性果蝇的平均寿命和半数死亡时间,其平均寿命延长率达41.84%;800mg/(kg·d)的茶树菇活性提取物能使雄性小鼠血清SOD活性提高42.36%,肝脂褐素含量减少50.06%,脾指数增加33.33%,脑指数增加17.85%;小鼠对茶树菇活性提取物的耐受量至少为20g/(kg·d)。结论茶树菇活性提取物具有良好的抗氧化和延缓衰老的效果,且毒副作用较小。

Objective To investigate the antioxidative and antiaging effects of the bioactive extract from Agrocybe aegerita. Method Fruit flies and D-galactose induced aging mice were fed with the bioactive extract from Agrocybe aegerita. The lifespan of fruit fly and spleen index, brain index, SOD activity in serum and lipofuscin level in liver of the mice were measured, and its acute toxic effect was studied. Results The bioactive extract from Agrocybe aegerita could significantly increase the mean lifespan and half death time of fruit flies. The percentage of life prolongation was 41.81% compared with the control. Compared to the models, SOD activity in serum, spleen index and brain index of male mice fed with 800mg/（kg·d） of the bioactive extract from Agrocybe aegerita were respectively increased by 42.36%, 33.33% and 17.85%, while lipofuscin content of liver decreased by 50.06%. 20g/（kg·d） of the bioactive extract from Agrocybe aegerita was safe for mouse. Conclusion The bioactive extract from Agrocybe aegerita have obviously antioxidative and antiaging effects with little side effect.