摘要
目的探讨番茄红素预防高脂喂饲大鼠脂代谢紊乱的作用及机制。方法用高脂饲料喂养大鼠,同时,给予番茄红素灌胃,检测血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、肝重、肝指数、脂蛋白脂酶(LPL)和肝脂酶(HL)活性;用蛋白印迹法测定低密度脂蛋白受体(LDL-R)的表达,探讨番茄红素预防高脂血症的可能机制。结果番茄红素各剂量组的血TC、TG、LDL-C肝重及肝指数显著减轻,有效地提高了LPL和HL活性,其中,高剂量组明显。同时,番茄红素可增加LDL-R的表达。结论番茄红素具有预防高脂喂饲大鼠肝组织脂代谢紊乱的作用。其机制可能是通过提高LRL和HL活性,增加LDL-R的表达,减少甘油三酯(TG)和低密度脂蛋白(LDL)在肝组织中的蓄积,从而预防肝组织脂代谢的紊乱。
Objective To study the protective mechanism of lycopene by modulating lipid metabolic disturbance in rats. Method The hyperlipidemic rats were induced by high fat diet and were divided into groups given different doses of lycopene. The serum total cholesterd （TC） , triglyceride （TG） , low density lipoprotein cholesterol （LDL-C） , high density lipoprotein cholesterol （HDL-C） , the hepatic lipase （HL） and lipoprotein lipase （LPL） activity in liver were tested by biochemical method, and the expression of low density lipoprotein receptor （LDL-R） in liver was detected by Western blot analyses. Results Lycopene decreased serum TC, TG LDL-C, and the activity of LPL and HL in three lycopene groups were higher than those in model group, and the high dose group was the highest. Conclusion Lycopene may increase the lipases＇activity and the expression of LDL-R, and decrease the deposit of TG and LDL in liver to prevent lipid metabolic disturbance.
出处
《营养学报》
CAS
CSCD
北大核心
2008年第3期307-310,共4页
Acta Nutrimenta Sinica
基金
教育部春晖计划资助资助项目(No.Z2005-271001)
