刺突蛋白亚单位基因疫苗在大鼠体内诱导的体液免疫反应
摘要
目的利用重组腺病毒表达SARS病毒蛋白N端，研究其在动物体内诱导的体液免疫反应，探讨将其进一步开发为SARS病毒基因疫苗的可行性。方法用截短的刺突蛋白S1亚单位（SS）腺病毒载体疫苗（Ad—SS）皮下免疫大鼠[1×10^7pfu／（鼠·次），每周1次，连续3周]，用ELISA法测定血清中抗SARS—CoV IgG水平。结果Ad—SS在大鼠体内能诱导产生高滴度的抗SARS冠状病毒IgGs，IgGs在免疫开始2周出现，在最后一次免疫1～2周达到最高（最高滴度为1：2^8.5）。用Vero—E6细胞体外攻击保护试验检测免疫动物血清中SARS病毒中和抗体发现，Ad—SN免疫鼠血清能在体外保护Vero—E6细胞免受SARS病毒攻击，中和滴度达到1：2^6.9。结论Ad—SN能在大鼠体内诱导SARS病毒特异的保护性体液免疫，有望发展成为一种SARS基因疫苗。
出处
《广东医学》
CAS
CSCD
北大核心
2008年第7期1132-1134,共3页
Guangdong Medical Journal
基金
广州市科技攻关计划重点资助项目(编号:2003Z2-F0025)
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