胃癌中幽门螺杆菌对hMSH2和hMLH1基因表达的影响

Effects of Helicobacter pylori on hMSH2 and hMLH1 Gene Expression in Gastric Carcinoma

目的:探讨胃癌中幽门螺杆菌﹙Helicobacter pylori,Hpylori﹚对hMSH2和hMLH1基因表达的影响。方法:利用尿素酶快速检验法确定胃癌组织及同病例癌旁粘膜、胃镜非癌患者胃粘膜组织有无Hpylori感染;应用免疫组织化学方法检测所有标本的hMSH2、hMLH1蛋白的表达。结果:胃癌组的hMSH2蛋白阳性表达率显著高于癌旁组和非癌组(P<0.05);而后两者无显著差别(P>0.05)。胃癌组、癌旁组和非癌组的hMLH1蛋白阳性表达率无显著差别(P>0.05)。胃癌组织中,Hpylori感染组的hMSH2蛋白阳性表达率和hMLH1蛋白阳性表达率均显著低于非感染组(P<0.05);癌旁组织中,Hpylori感染组的hMSH2蛋白阳性表达率和hMLH1蛋白阳性表达率均显著低于非感染组(P<0.05);非癌患者胃粘膜中,Hpylori感染组与非感染组的hMSH2蛋白阳性表达率及hMLH1蛋白阳性表达率均无显著差别(P>0.05)。结论:胃粘膜hMSH2蛋白表达上调并hMLH1蛋白表达下调可能是胃癌发生的标志;Hpylori感染导致的胃粘膜细胞hMSH2和hMLH1蛋白表达降低,可能是促进胃癌发生的一个因素。

Objective： To investigate the effect of Helicobacter pylori （H. pylori） on hMSH2 and hMLH1 gene expression in gastric carcinoma. Methods： The ultra-quick urease test was used to detect H. pylori in gastric carcinoma samples, corresponding adjacent mucosa and non-carcinoma gastric mucosa. Immunohistochemistry was employed to observe hMSH2 and hMLH1 expression. Results： The positive rate of hMSH2 protein in gastric carcinoma tissues was significantly higher than that in carcinoma-adjacent mucosa and non-carcinoma mucosa（P〈0.05）, with no significant difference between the latter two groups（P〉0.05）. No statistical significance was found in the positive rate of hMLH1 protein expression among gastric carcinoma tissues, carcinoma-adjacent mucosa and non-carcinoma mucosa（P〉0.05）. In the gastric carcinoma tissues, the positive rates of hMSH2 protein and hMLH1 protein detection in those with H. pylori infection were significantly lower than in those without H. pylori infection（P〈0.05）. In the carcinoma-adjacent mucosa, the positive rates of hMSH2 protein and hMLH1 protein in those with H. pylori infection were significantly lower than those without H. pylori infection（P〈0.05）. In the non-carcinoma mucosa, no significant difference was found in the positive rates of hMSH2 protein and hMLH1 protein between those with and without H. pylori infection（P〉0.05）. Conclusion： Upregulation of hMSH2 protein and downregulation of hMLH1 protein may be potential markers for gastric carcinoma. Decreased hMSH2 and hMLH1 protein expression induced by H. pylori infection may promote gastric carcinogenesis.