摘要
目的通过体外诱导研究大鼠骨髓间充质干细胞向心肌样细胞的分化,为心衰的干细胞移植治疗提供实验依据。方法分离培养大鼠骨髓间充质干细胞,采用5-氮杂胞苷定向诱导,通过形态学、免疫细胞化学及磷钨酸苏木素染色(PTAH)鉴定。结果诱导后细胞以梭形、柱状为主;部分细胞有分支,核一个、卵圆形、居中,类似心肌细胞形态;诱导24h后培养2周,Sarcomeric Actin和Connexin-43表达较弱,以后表达逐渐增强;诱导细胞PTAH阳性。结论骨髓间充质干细胞能在体外被诱导分化为心肌样细胞,有望成为心衰时干细胞移植治疗的理想细胞材料。
Objective To study the differentiation of rat marrow-derived mesenchymal stem cells （MSCs） to cardiomyocytes-like in vitro via induction and provide experimental foundation for serving MSCs as a new cell source in stem cell transplantation for heart failure therapy. Methods Rat MSCs were isolated and cultured, then induced with 5-azacytidine for 24 h. After cultured for additional 1, 2, 3 and 4 weeks, the cells were identified by morphology, immunocytochemistry and staining of phosphotungstic acid hematoxylin （PTAH）. Results Most MSCs induced by 5-azacytidine became spindle-like and pillar-form with oval nuclei lying in the center of cells and similar to cardiomyocytes. After more than 2 weeks of cultivation, positive staining of Sarcomeric Actin and Connexin-43 were observed on MSCs induced by 5-azacytidine. In addition, positive staining of PTAH was identified on MSCs induced by 5-azacytidine. Conclusion MSCs can differentiate into cardiomyocytes in vitro after induced by 5-azacytidine, which suggests that MSCs might be a new cell source of stem cell transplantation in heart failure therapy.
出处
《解剖学研究》
CAS
2008年第3期161-164,共4页
Anatomy Research
