广东汉族人群LMP和TAP基因多态性及与变应性鼻炎的相关性

Association between gene polymorphism of transporters associated with antigen processing, low molecular weight peptide and allergic rhinitis in a Guangdong population

目的对广东地区汉族变应性鼻炎患者作抗原处理相关肽(TAP)和低分子量多肽(LMP)分型,探讨这些基因与广东人群中变应性鼻炎遗传易感性的可能关系。方法采用PCR扩增阻碍突变系统(PCR-ARMS)法和PCR-RFLP法对62名无亲缘关系的变应性鼻炎病人和95名无血缘关系的广东籍健康汉族人作TAP、LMP分型。结果TAPl-333、637位等位基因基因型在广东汉族变应性鼻炎组及正常对照组中均以I和D为主,TAP2-379、565、665等位基因基因型分别为V-A-T。TAP1和TAP2各等位基因基因型频率在变应性鼻炎组和正常人组间差异无显著性(P>0.05)。变应性鼻炎患者LMP7A/A频率占8.70%,低于正常对照组的21.35%,有显著差异(P<0.05),其余各型与正常对照组无明显差异(P>0.05)。变应性鼻炎病人中LMP7等位基因A的频率占36.23%,B的频率占63.77%,与正常对照组的A为47.09%、B为52.91%有显著差异(P<0.05)。结论提示LMP7等位基因A与AR的发病呈负关联,B与AR的发病呈正关联。变应性鼻炎与TAP基因可能无相关性.

Objective To investigate the distribution of transporter of antigen peptides （TAP） genes and low molecular weight peptide in a Guangdong population and to explore the association of these genes with allergic rhinitis. Methods Ninetytwo unrelated healthy individuals of Han nationality and 69 patients with allergic rhinitis who came from the same geographic region were enrolled in the study. The TAP was genotyped by PCR-ARMS technique and LMP by the PCR- RFLP method Results Six TAP1 and 6 TAP2 alleles were observed. TAP1 A was the most common allele of the TAP1 locus, and TAP2 A was the most common allele of the TAP2 locus. There was no significant difference in allelic frequencies of TAP1, TAP2 gene between patients and normal controls. There was significant increase of LMPTB and a significant decrease of LMPTA allelic frequency in patients with allergic rhinitis compared with controls, No significant differences in allelic frequencies of LMP2 gene between the patients and normal controls were found, Conclusion LMP7 is strongly associated with allergic rhinitis, The polymorphisms of antigen processing genes may not associate with allergic rhinitis.