树突状细胞活化的CTLs联合5-FU在裸鼠体内外对喉癌的抑制作用

Inhibitive effect of CTLs actived by dendritic cells associated with 5-FU on laryngocarcinoma in vitro and in nude mice

目的探讨树突状细胞(DCs)活化的细胞毒T细胞(CTLs)联合5-氟尿嘧啶(5-FU)在裸鼠体内外对喉癌的抑制作用。方法MTT法检测DCs激活的CTLs联合5-FU在裸鼠体外对喉癌细胞的抑制作用。裸鼠体内实验分为CTLs治疗组、5-FU治疗组、CTLs/5-FU治疗组及对照组,观察喉癌移植瘤的成瘤率、潜伏期、瘤重及瘤体积。结果治疗组在裸鼠体外都能显著抑制喉癌细胞生长,以CTLs/5-FU治疗组的效果最为显著(P<0.01)。在裸鼠体内实验中,治疗组的成瘤率、瘤体积、瘤重都显著降低,潜伏期延长,以CTLs/5-FU治疗组最为显著(P<0.01)。结论P联合应用DCs激活的CTLs及5-FU比单独应用CTLs或5-FU对喉癌的抑制效果更佳。

Objective To investigate the inhibitive effect of cytotoxic T lymphocytes （CTLs） actived by dendritic cells （DCs） associated with 5-Fluorourac（5-FU） on laryngocarcinoma in vitro and in nude mice. Methods The inhibitive effect of CTLs actived by DCs associated with 5-FU on laryngocarcinoma was detested in vitro by MrI＇F methods. In addition, there were four groups in experiments of nude mice： control group, CTLs group, 5-FU group and CTLs/5-FU group. These treatements were given before or after the mice were attacked by laryngocarcinoma cells and the tumor generating rate, the latent phase, the volumn and weight of tumor were observed. Results The inhibitive effect of three therapy groups was significantly increased, especially the CTLs/5-FU group in vitro （P〈0.01）. Additionally, in nude mice, the latent phase of therapy groups were increased and the tumor generating rate, the volume and weight of tumor were decreased, especially in the CTLs/5-FU group （P〈0.01）. Conclusion CTLs actived by DCs associated with 5-FU are more effective to laryngocarcinoma than CTLs or 5-FU.