图形化表面TiO_2薄膜的制备及其对内皮细胞附着形态的影响

Preparation of the topographed-TiO_2 films and the influence on the adhesion behavior of endothelial cells

采用光刻技术和反应离子刻蚀法(RIE)先在硅表面制备出一系列平面尺寸不同的微图形阵列,然后采用溶胶凝胶(sol-gel)浸渍提拉法在其表面制备出均匀致密、表面粗糙度一致的锐钛矿型纳米晶TiO2薄膜。利用扫描电镜(SEM)和免疫荧光标记实验研究图形尺寸对人脐静脉内皮细胞(HUVEC)附着形态的影响。实验结果发现在化学成分、细胞毒性、表面粗糙度都不对细胞附着形态产生影响的条件下,HUVEC对图形尺寸的响应为:当平面空间足够时细胞的附着伸展性能较好,而当没有足够的平面空间时细胞的附着伸展性能较差。

Surfaces topographies on Si wafer substrates with different dimesions have been produced by photolithography and reactive ion etching（RIE）,and subsequently titanium dioxide films were coated on them via sol-gel method.The coated TiO2 films with the same roughness have an anatase-typed nano-structured,and they are compact and homogeneous.The adhesion behavior of human umbilical vein endothelial cells（HUVEC） on the photograhpied-TiO2 films were investigated by means of scanning electron microscopy（SEM） and fluorescent staining.It is found that the differences of cell adhesion behavior on sample surfaces are predominantly attribute to the dimesions of the topographies because the chemical composition,cytotoxicity,surface roughness of the surfaces are quite similar.It seems that the cells exhit a better adhesion state on the sufaces on which larger flat-area is applied for the cells to stretch themselves.