薄层扫描法测定强骨颗粒中黄芪甲苷的含量

Using TLCS to Determine the Contents of Astragaloside Ⅳ in QiangGu Granula

采用双波长薄层扫描法，以氯仿-甲醇-水（13：7：2）的下层溶液为展开剂，10％硫酸乙醇溶液显色，在105℃烘约5min，k=530nm，k=700nm条件下扫描，以测定强骨颗粒中黄芪甲苷含量．结果表明：样品中黄芪甲苷分离完全，阴性供试品无干扰，黄芪甲苷在1．982～9．91μg范围内呈良好线性关系，r=0．9957，平均回收率为98．4％，RSD为2．1％，测得3批强骨颗粒中黄芪甲苷的平均舍量为0．0639mg/g。

Objective：To establish an assaying method for QiangGu granula using TLCS. Method： Applying scanner to determine the contents of astragaloside IV. The detection wavelength is λs = 530 nm, λR = 700 nm; the developing agent is under layer of chloroform-methanol-water（113：7：2） ;the developer is mixed solution of 10% sulfuric acid and alcohol. Results：The astragaloside Ⅳ of sample is completely separated. The Linearity relation of astragaloside Ⅳ is good during 1.982 ～9.91 μg, r = 0.9957. The average recovery is 98.4% ,RSD = 2.1% .The average contents of three batches of QiangGu granula is 0.0639 mg/g.