高产共轭亚油酸植物乳杆菌的诱变选育

Mutation Breeding of Conjugated Linoleic Acid Producing Strain with Lactobacillus plantarum

利用人工诱变方法选育高产共轭亚油酸生产菌株,以实验室保藏的植物乳杆菌Lactobaci-llus plantarum A(简写为L.plan A)为出发菌株,经紫外线,硫酸二乙酯的依次处理,结合高浓度亚油酸平板(0.1%)的筛选和进一步摇瓶复筛,最终得到一株共轭亚油酸高产菌株Lactobacillus plantarum H3-1(简写为L.plan H3-1),其共轭亚油酸产量高达30.67 mg?L-1,比出发菌株L.plan A提高了264.5%.传代实验中突变株L.plan H3-1的遗传特性较稳定.实验结果证明该诱变方法对提高植物乳杆菌L.plan A的共轭亚油酸产量效果比较显著.

A mutant of high yield of conjugated linoleic acid （CLA） was obtained by inducing mutation methods. A parent strain of Lactobacillus.plantarum A（L.plan A）was treated by UV and chemical mutagen ehyl sulfate in an alternate fashion, and combined with the method of high concentration（0.1%） substrate of linoleic acid screening. A strain named Lactobacillus.plantarum H3-1（L.plan 3-1）, overproducing conjugated linoleic acid, was obtained by shaking flask fermentation, The yield of CLA reached 30.67mg·L^-1 with the mutant, which was 264.5 %. higher than parent strain. Pass-generation test indicate that the hereditary properties of the mutant are stable. Experimental results demonstrate that the abundant production of CLA of the mutant can be realized by applying such proposed mutation methods.