芽孢杆菌K1降解亚硝酸盐的特性研究

Studies on degradation of nitrite by Bacillus K1

研究了芽孢杆菌K1对NO2-N的降解作用。对影响降解的主要因素如NO2-N初始浓度、培养时间、接种量、装液量、静置与摇床培养等进行实验,结果表明:K1在NaNO2初始浓度为500mg/L的营养肉汤培养48 h后,NO2-N盐降解率最高,达到了98.92%。随着培养时间的延长,NO2-N降解率升高,在K1菌生长对数期,NO2-N降解速度最快。24和48 h时,接种量为0.5%的NO2-N降解率低于接种量大于1%的降解率,说明了培养液初始菌量的大小对NO2-N的降解有影响。NO2-N降解率随着装液量的减少而降低。K1静置培养的菌量和NO2-N降解率都低于摇床培养的菌量和NO2-N降解率。

The process that bacillus K1 degraded nitrite was studied. The experiment conduct by the initial concentration of nitrite, culture time, inoculum size, dissolved oxygen. The result showed that the best degradation rate is 98.92 %, when the culture in nutri- ent broth 48h with the concentration of NaNO2 was 500mg,/L. With culturing time prolong, the nitrite degradation rate run up, when K1 got growth the logarithmic phase, degradation speed was the rapidest. When 24h and 48h, nitrite degradation rate of 0.5% inoculum size groups were all below the more than 1% groups. It showed that the nitrite degradation rate was affected by the initial inoculum size. With injection volume decreasing, the degradation rate of nitrite could be reduced. The K1 concentration and nitrite degradation rate were lower in the static culture than shake culture.