摘要
对强弱毒力不同的核盘菌Ep-1PNA5和Ep-1PN的主要致病因子草酸和果胶酶的产生进行了比较研究。结果发现强毒力的Ep-1PNA5和弱毒力Ep-1PN都可产生草酸,并且在发病油菜活体组织上,弱毒力Ep-1PN的病组织中的草酸含量高于Ep-1PNA5病组织;两个菌株在发病油菜活体组织上的果胶酶产量没有差异,但在诱导培养基中Ep-1PN菌株比Ep-1PNA5的果胶酶产量高。这一结果表明弱毒力Ep-1PN菌株毒力的衰退并不是因为其所携带的dsRNA因子抑制了草酸和果胶酶所产生的。
The production of oxalic acid （OA） and polygalacturonase （PG） by S. sclerotiorum strains Ep - 1PN and Ep - 1PNA5 was compared. The results showed that both strains could normally produce OA and PG, and production of oxalic acid by Ep - 1PN was larger than that by Ep - 1PNA5 on living rape leaves. The production of PG by two strains on living rape leaves had no difference, but the production of PG by Ep - 1PN was larger than that by Ep - 1PNA5 on inducing medium. The results also showed that the recession of virulence of Ep - 1PN is not because that the dsRNA factor in Ep - 1PN inhibited the production of oxalic acid and PG.
出处
《氨基酸和生物资源》
CAS
2008年第2期5-8,共4页
Amino Acids & Biotic Resources
基金
公益性行业(农业)科研专项资助项目.nyhyzx-07-0547
关键词
核盘菌
毒力
草酸
果胶酶
Sclerotinia sclerotiorum
virulence
oxalic acid
polygalacturonase
