反相高效液相色谱法测定万寿菊中的玉米黄质

Determination of Zeaxanthin in Marigold by RP-HPLC

用反相高效液相色谱法测定万寿菊中的玉米黄质。以丙酮与石油醚提取色素，色谱柱Kromasil（C18 4．6mm×150mm），乙腈：甲醇：二氯甲烷（体积比为70：20：10）为流动相，在450nm下检测玉米黄质。结果表明，玉米黄质的质量分数为0．158％，玉米黄质的回收率是94．7％～98．1％，平均回收率为96．4％（RSD=1．36％）。该方法简便、准确，有良好的重现性，技术参数指标符合食品理化分析的要求。

A reversed -phase high performance liquid chromatographic method was used to determine zeaxan- thin in marigold. Zeaxanthin was extracted from marigold with acetone and petroleum ether. Using Kromasil C18 column（4.6 mm × 150 mm） , ace-tonitrile： methanol： methylene chloride （70：20：10） as mobile phase, zeaxanthin was eluted and monitored at 450nm. It was shown that the content was 0. 158%, the recoveries were 94.7 % - 98.1% and the average recoveriy was 96.4% （ RSD = 1.36% ）. This method is simple, accurate and reproducible.