高蛋白饮食对犬加替沙星药动学的影响研究

Effects of High Protein Diet on Pharmacokinetics of Gatifloxacin on Dogs

目的:研究高蛋白饮食对犬加替沙星药动学的影响。方法:8只Beagle犬随机交叉空腹或给予高蛋白饮食同时单次口服加替沙星胶囊17mg·kg-1,采用高效液相色谱法测定血药浓度,用3p97软件计算两者的药动学参数并比较评价。结果:空腹组和高蛋白饮食组的药-时曲线符合口服吸收一室摸型,主要药动学参数分别为t1/2ke(4.00±0.84)、(9.28±1.32)h;tmax(2.29±1.08)、(4.98±2.76)h;Cmax(6.06±0.87)、(3.42±1.20)μg·mL-1;AUC(0～t)(56.74±10.80)、(68.66±15.92)μg.h.mL-1,2组比较各参数均有显著性差异(P<0.05或P<0.01)。结论:高蛋白饮食明显影响犬加替沙星药动学参数。

OBJECTIVE： To study the effect of high protein diet on pharmacokinetics of gatifloxacin. METHODS： A randomized crossover design was performed in 8 Beagle dogs. In the two study periods, a single oral 17 mg · kg^-1 dose was administered to empty stomach or with high protein diet in dogs. Plasma concentration of gatifloxacin were measured by HPLC. The pharmacokinetic parameters were calculated by 3p97. RESULTS： The concentrationtime curves were a one compartment model in empty stomach or with high protein diet in beagle dogs. The t 1/2ke were（4.00± 0.84） and （9.28 ± 1.32） h, tmax（2.29±1.08） and （4.98±2.76） h, C （6.06±0.87） and （3.42±1.20） μg·mL^-1, AUC（0-t） （56.74±10.80） and （68.66± 15.92） μg · h · mL^-1, respectively. There was significant difference between the two groups in the main pharma- cokinetic parameters（P 〈0.05 or P 〈 0.01）. CONCLUSION ： The high protein diet can influence pharmacokinetic parameters of gatifloxacin.