微小剂量阿司匹林对高尿酸血症模型小鼠血尿酸的影响考察

Effect of Minidose Aspirin on Uric Acid in Hyperuricemia Model Mice

目的:考察微小剂量阿司匹林对高尿酸血症模型小鼠血尿酸水平等的影响。方法:75只小鼠随机分为5组,包括正常对照组、高尿酸对照组和阿司匹林低、中、高实验组。对照组给予0.5%羧甲基纤维素钠溶液,实验组分别给予3种剂量(12.5、25、50mg·kg-1)阿司匹林溶液,每天1次,持续1周后,除正常对照组外,其余4组腹腔注射尿酸溶液,建立高尿酸血症模型,并于建模后1h取血样测定尿酸、肌酐和尿素氮水平。结果:与高尿酸对照组比较,各剂量阿司匹林对肌酐、尿素氮影响不明显,但可显著升高血尿酸水平(P<0.0001),但随剂量增加,血尿酸水平逐渐降低。结论:微小剂量阿司匹林可导致高尿酸血症模型小鼠血尿酸水平升高,推测其作用机制可能与抑制尿酸排泄有关。

OBJECTIVE： To evaluate the effects of minidose aspirin on blood uric acid, creatinine and urea nitrogen in hyperuricemia model mice. METHODS： 75 mice were randomly assigned to receive 0.5% CMC- Na （normal control group and hyperuricemia control group） or aspirin （12.5, 25, 50 mg· kg^-1 · d^-1） q.d for 1 week. Then all the mice except the normal control group were intraperitoneally administered with uric acid to establish hyperuricemia model. 1 h later, the blood sample was collected for determination of uric acid, creatinine and urea nitrogen. RESULTS： As compared with hyperuricemia control group, mini- dose aspirin had no apparent effect on creatinine and urea nitrogen, but remarkably increased the level of blood uric acid（P 〈 0.000 1）, and the level blood uric acid decreased in a dose - dependent manner. CONCLUSIONS： Minidose aspirin can significantly increase blood uric acid level in hyperuricemia model mice, and this effect might attribute to its inhibition on the excretion of uric acid.