周围淋巴结地址素和血管细胞粘附分子-1在妊娠7d小鼠淋巴结和子宫的表达

Study of Peripheral Lymph Node Vascular Addressin and Vascular Cell Adhesion Molecule-1 in C57Bl/6 Mice at Gestation day 7

目的:研究正常小鼠妊娠第7 d子宫和淋巴结的淋巴细胞归巢分子[周围淋巴结地址素(PNAd)和血管细胞粘附分子-1(VCAM-1)]的分布。方法:应用免疫组化染色观察C57Bl/6小鼠妊娠第7 d PNAd和VCAM-1在子宫和淋巴结的表达。结果:在小鼠妊娠第7 d中,PNAd强表达于外周淋巴结高内皮静脉血管的内皮细胞,但在子宫,仅弱表达于子宫内膜上皮;VCAM-1弱表达于淋巴结部分血管内皮、淋巴细胞,但广泛表达于子宫的各层血管内皮。结论:PNAd和VCAM-1在小鼠妊娠第7 d子宫和淋巴结有各自的分布特点,可能与妊娠期淋巴细胞的分布有关。

Objective： To detemaine the distribution of lymphocyte-homing molecules[ peripheral lymph node vascular addressin（PNAd） and vascular cell adhesion molecule-1 （VCAM-1）] in the lymph node and uterus of C57B1/6 mice at gestation day 7. Methods： Immunohistochemistry assay was applied to observe the expression of PNAd and VCAM-1 in the lymph node and uterus of C57B1/6 mice at gestation day 7. Results： PNAd was strongly expressed in the endothelial cells of high endothelial venules, but weakly expressed in the endometrium epithelial cells. VCAM-1 was weakly expressed in the parts of vessel endothelium cells and lymphocytes in the lymph node, but expressed widely in all kinds of vessel endothelium in the uterus. Conclusion： The distribution of PNAd and VCAM-1 in the lymph node and uterus of C57Bl/6 mice at gestation day 7 is supposed to be related to the distribution of lymphocytes in the gestation.