脂糖舒对D-半乳糖衰老模型小鼠组织SOD活性和MDA含量的影响

Effects of Zhitangshu on SOD Activity and MDA Level in Tissues of Aged Mice Induced by D-galactose

目的观察脂糖舒对D-半乳糖衰老模型小鼠组织超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量的影响。方法采用D-半乳糖制造小鼠衰老模型,同时灌胃不同剂量脂糖舒(Ⅰ组5g/kg、Ⅱ组10g/kg,1次/d),连续6周,并取健康小鼠10只作正常对照。6周末检测小鼠心、肝、肾和脑组织中SOD活性和MDA含量。结果模型组与正常组比较,小鼠各组织中SOD活性明显降低,MDA含量明显升高,P<0.05或P<0.01;而脂糖舒两剂量组较模型组小鼠心、肝、肾组织中SOD活性明显升高,MDA含量明显降低,P<0.05或P<0.01;脂糖舒两剂量组间的SOD活性和MDA含量无显著性差异(P>0.05)。结论脂糖舒能通过提高抗氧化能力来发挥延缓衰老的作用。

Objective To observe the effects of Zhitangshu（ZTS） on the superoxide dismutase （SOD） activivty and malondialdehyde（MDA） level in tissue of aged mice. Methods The aged model was induced by D-galactose,then they were treated intragastricaUy with ZTS at different doses （ Ⅰ group：5g · kg^-1 · d^-1,Ⅱ group：10g · kg^-1 · d^-1）for six weeks. Other ten mice were used as control. At the end of sixth week, the SOD activity and MDA content in tissues of heart,liver, renal and brain of mice were mesaured. Results The activity of SOD was lower and the content of MDA was higher in aged model group than those in control group （P 〈 0. 05 or P 〈 0.01 ） ; Compared with aged model group, the activities of SOD were increased and the contents of MDA in tissues of heart,liver and renal of mice were significantly reduced in low and high dose ZTS groups （P 〈0.05 or P 〈0.01 ）. Conclusion ZTS has an anti-aging effet by increasing SOD actirity and inhibiting tipid perosidation.