热休克蛋白60及其抗体在扩张型心肌病患者中的表达

Study on HSP 60 and Its Antibody in Patients with Dilated Cardiomyopathy

目的探讨扩张型心肌病(DCM)心肌组织中HSP60以及血清中抗人HSP60抗体在DCM发生、发展中的作用。方法应用免疫组织化学方法检测15例DCM患者及7例正常对照者心肌组织中HSP60的表达和分布;采用酶联免疫吸附试验(ELISA)法检测44例DCM患者和42例对照者血清中的抗人HSP60抗体水平。结果DCM患者心肌组织中HSP60的表达明显增加,HSP60几乎全部为胞浆性表达,未见核阳性颗粒。DCM患者血清中的抗人HSP60抗体水平较对照组明显升高(P<0.05)。结论DCM患者HSP60在心肌组织的过高表达及其血清抗体水平的升高,可能促进DCM的发生、发展,同时又为心肌提供一定的保护作用。

Objective To explore the roles of heat shock protein 60 （ HSP60 ） in myocardial tissue and serum anti-human HSP60 antibody in the initiation and progression of dilated eardiomyopathy （DCM）. Methods The levels of HSP60 in myocardial tissue in 15 DCM patients and 7 controls were detected by immunohistochemical staining. The serum anti-Hsp60 antibody levels in 44 DCM cases and 42 controls were measured by enzyme-linked immunosorbent assay（ELISA）. Results The expression of HSP60 increased significantly in the DCM hearts compared to the control hearts （ P 〈 0.05 ）. Most of the HSP60 were observed in cytosol other than in nucleus or membrane. In comparison with the control group, there was an obvious increase in the serum anti-Hsp60 antibody levels of the DCM group （P 〈 0.05 ）. Conclusion The overexpression of HSP60 in DCM myocardial tissue might contribute to the initiation and progression of DCM, and meanwhile afford some protective effects on myocardial response to stress. Maybe the elevated serum anti-HSP60 antibody level has the similar effect.