特发性血小板减少性紫癜患者外周血调节性T细胞的研究

The research of peripheral blood regulatory T cell in patients with idiopathic thrombocytopenic purpura

目的研究特发性血小板减少性紫癜(ITP)患者外周血CD4+CD25+调节性T细胞(Tr细胞)的变化,探讨其在ITP发病机制中的作用。方法分别收集35例ITP患者及健康对照组外周抗凝静脉血,分离纯化T淋巴细胞。应用藻红蛋白(PE)标记的抗CD4单抗及异硫氰酸荧光素(FITC)标记的抗CD25单抗,作双色流式细胞术,分析ITP患者外周血CD4+CD25+调节性T细胞百分率。结果ITP组外周血中Tr细胞明显低于健康对照组(P<0.05),Tr细胞占CD4+T细胞的比例也低于健康对照组(P<0.05)。结论ITP患者外周血存在细胞免疫功能失调,Tr细胞数量减少可能与ITP的发病机制有关。

Objective To investigate the change of peripheral blood CD4^＋ CD25^＋ regulatory T cells （Tr） in patients with idiopathic thrombocytopenic purpura （ITP）, and to analyze its role in the pathogenesis of ITP. Methods Anticoagulated venous blood was collected from ITP patients （ITP group,n=35） and healthy controls （healthy control group,n=35）. T lymphocytes were isolated and purified with human CD3^＋ T cell enrichment columm. The percentage of peripheral blood CD4^＋ CD25^＋ regulatory T cells was detected by immunofluorescence staining （PE-anti-CD4 monoclone antibody and FITC-CD25 monoclone antibody） and bicolor flow cytometry by CD25/CD4 gating. Results The number and constituent ratio of CD4^＋ CD25^＋ T cell were significantly lower in ITP group than those of healthy control group （P〈0.05）. Conclusion There is peripheral blood celluar immunological function disorder in ITP patients, and decrease of CD4^＋ CD25^＋ T cell population may be involved in the pathogenesis of ITP.