犬MC2R基因5′-UTR、3′-UTR序列测定、分析及其5′侧翼启动区序列分析

Molecular Cloning and Sequence Analysis of Melanocortin2-Receptor Gene in Beagle Dog

目的分离犬MC2R基因CDS部分序列和该基因5′、3′非翻译区,并对5′、3′非翻译区和5′侧翼的启动区进行分析。方法本研究采用反转录PCR(RT-PCR)和RNA连接酶介导的RACE(RLM-RACE)技术和BLAST分析软件。结果得到了5′、3′非翻译区和部分CDS片段的序列,他们的大小分别为168bp、1366bp和987bp,并预测了大约1500bp的5′侧翼的启动区域。结论对它们进行分析显示,该基因至少由两个外显子(exon1和exon2)组成,exon1和exon2的一部分编码5′非翻译区(5′-UTR),exon2其余的部分编码整个编码区。其启动区有inr,SF-1,SP1,CRE,PPRE,AP-1等多个顺式作用元件,这些为犬MC2R表达调控研究提供研究奠定基础。

Abstract： Objective To isolate the partial CDS and 5′, 3′ - UTR and partial CDS of MC2R gene in dogs. Methods RT - PCR and RLM - RACE are used in this research, the BLAST analysis tool was also used. Results The 5′, 3′ - UTR and partial CDS has the lengh 168 bp, 1 366 bp and 987 bp respectively. About 1 500 bp of promoter region was predicted according to the fragment. Conclusions Two exons and the lengths of each exon and intron were found. Several kinds of cis - acting elements and its amounts have been analyzed.