利用糖蜜废液培养微生物絮凝剂

On the Production of a Bioflocculant Using Molasses

糖蜜废液含有大量的糖类和氨基酸等营养物质,不加入其他营养成分的条件下,将糖蜜废液稀释50倍,初始pH调至5,在150 r/min 30℃摇床培养絮凝剂产生菌HHE-P7,3 d后可得到微生物絮凝剂粗品的产量为3.1 g/L.絮凝剂中95%的活性成分存在于培养液的上清液中;高岭土悬浊液的pH、絮凝剂投加量以及Ca2+的添加量对絮凝效果都有影响.

Molasses waste is used as the culture medium because it＇ s abundant of nutrition substances such as saccharide and amino acid. When the molasses was diluted by 50 times, the initial pH was adjusted to 5.0, and the rotation speed was set 150 r/min at 30 ℃, the bioflocculant production reached 3.1 g/L after 3 d of cultivation. 95 % of activated substances were located in the supernatant. Kaolin suspension pH, feed volume of the flocculant and Ca^2＋ exhibit effects on the flocculating activity.