姜黄素通过Notch途径抑制人宫颈癌细胞株HeLa细胞增殖

Inhibition of curcumin on the proliferation in HeLa cell lines through Notch signaling pathway

目的:探讨姜黄素对人宫颈癌细胞株HeLa细胞体外增殖抑制及凋亡诱导作用及相关机制。方法:MTT法检测不同浓度姜黄素对HeLa细胞的增殖抑制作用;RT-PCR法检测HeLa细胞Notch1、Notch2、Jagged1、Bcl-2、Bax基因表达情况。结果:姜黄素对HeLa细胞具有增殖抑制作用,且呈时间-剂量依赖性。随着姜黄素剂量的增加,Notch1、Notch2、Bcl-2mRNA的表达逐渐下降,Bax mRNA的表达逐渐升高,Bcl-2/Bax亦逐渐减小,Jagged1mRNA无明显变化。结论:姜黄素可能通过Notch信号途径改变凋亡蛋白的表达,抑制HeLa细胞增殖。

Objective： To investigate the regulatory effect of curcumin on the proliferation of human cervical carcinoma cell llne HeLa in vitro and its mechanisms. Methods： The antiproliferative effects of curcumin against HeLa cell lines was determined by the MTT dye uptake method. The expression of Notch1, Notch2, Jaggedl, Bcl - 2, Bax mRNA were evaluated by reverse transcriptnse - polymerase chain reaction （ RT - PCR） analysis. Results： Curcumin inhibited the growth of HeLa cells on a time - and dose - depending manner. Curcumin downregulated the constitutive expression of Notch1, Notch2, Jaggedl, Bcl -2 genes, but upregulated the expression of Bax and the ratio of Bcl-2/Bax decreased, there was no significance in the expression of Jaggedl mRNA. Conclusion： Curcumin can inhibited the proliferation of HeLa cell lines through regulating expression of apoptotic protein related with Notch signaling pathway.