微纳尺度SiO_2对雄性大鼠生殖功能及子代的影响

EFFECT OF MICRO-NANO-SCALE SiO2 ON MALE REPRODUCTIVE ABILITY AND OFFSPRINGS IN RATS

目的探讨微米与纳米尺度SiO2染毒对雄性大鼠胚胎发育及子代的影响。方法将30只雄性Wistar大鼠分为5组，经气管分别注入不同浓度的纳米SiO2（20～40nm）与微米SiO2（1～10μm）。雄鼠染毒5周后与正常成年雌鼠按1：2合笼交配5d，于妊娠第20天各组处死一半雌鼠，检查胎鼠情况；另一半雌鼠自然分娩，检查仔鼠情况。结果纳米SiO2对雄性大鼠染毒影响了大鼠交配及胚胎形成，纳米高剂量组雄鼠交配率（66．7％）及每窝活胎数（11．8）均小于对照组（100％、14．0），差异有显著性（P〈0．05）；但对胎鼠及仔鼠生长发育未产生明显影响，各组胎鼠及仔鼠体质量、身长等指标与对照组比较差异均无显著性（P〉0．05）。相同剂量纳米组与微米组比较，差异均无显著性（P〉0．05）。结论纳米SiO2对雄性大鼠生殖有一定的损伤作用，但对于成功受孕后的胎鼠及仔鼠影响不明显。

Objective To explore the effects of micm-nano-scale SiO2 on male reproductive ability and offsprings in rats. Methods Male Wistar rats were exposed to nano-meter SiO2（20 - 40 nm） and micro-meter SiO2（ 1 - 10 /an） by intratracheal injection once every other day for 5 weeks. The exposed male rats were matched with normal female rats in the ratio of 1：2 after exposure. On the 20 th day of the pregnancy, half of the female rats were sacrificed and the fetuses were examined. The offsprings of the rest female rats after normal labor were also examined. Results The mating rate and number of fetus in high dose nm-SiO2 group（66.7%, 11.8 respectively） were lower than those in control group （ 100%, 14.0）, （ P 〈 0.05 ）, but the development of fetus and offspring, including body weight and height, in exposed groups showed no significant difference from that in control group（ P 〉 0.05）. There were no significant differences in nm-SiO2 and μm-SiO2 at the same concentration（ P 〉 0.05）. Conclusion Nano-meter-silicon dioxide can affect male reproductive ability to some extent but has no significant effects on the fetuses when female rats are pregnant successfully.