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K562及其耐药细胞株K562/A02对rmhTRAIL的敏感性及NF-κB表达 被引量：1

Sensitivity of Cell Line K562 and Its Resistant Cell Line K562/A02 on rmhTRAIL and NF-κB Expression in the Cell Lines
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摘要 [目的]探讨K562及其耐药细胞株K562/A02对rmhTRAIL敏感性及NF-κB(RelA/p65)的表达。[方法]观察rmhTRAIL作用后K562、K562/A02细胞形态变化,采用碘化丙啶染色法流式细胞术定量检测细胞凋亡比例,MTT法测定rmhTRAIL抑制细胞增殖率,半定量逆转录—聚合酶链反应(RT-PCR)方法检测K562、K562/A02细胞的NF-κB(RelA/p65)mRNA表达水平。[结果]rmhTRAIL可诱导K562、K562/A02细胞凋亡,有典型的细胞形态改变;流式细胞术检测显示K562/A02的sub-G1百分比大于K562(P<0.05);rmhTRAIL对K562/A02的增殖抑制作用优于K562(P<0.05);K562和K562/A02均高表达NF-κB(RelA/p65)mRNA,两者无统计学意义(P>0.05)。[结论]rmhTRAIL可以诱导K562、K562/A02细胞凋亡;rmhTRAIL对K562/A02促凋亡和抑制增殖作用优于K562;NF-κB(RelA/p65)未显示出其在K562及其耐药细胞株K562/A02对rmhTRAIL敏感性差异中的作用。
作者 王雅茹 张学军 田艳 张兰凤 王志宇 栾天燕 韩强 毕雪冰
机构地区 保定市第三医院 河北医科大学附属二院
出处 《肿瘤学杂志》 CAS 2008年第6期473-476,共4页 Journal of Chinese Oncology
关键词 肿瘤坏死因子 NF-ΚB 细胞株 K562 基因 MDR 细胞凋亡
分类号 R965 [医药卫生—药理学]
  • 相关文献

参考文献7

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二级参考文献12

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同被引文献19

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肿瘤学杂志
2008年 第6期

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