α-氰基-N-苄苯乙烯胺对子宫内膜癌细胞增殖和凋亡的影响

The Effect of AG490 on the proliferation and apoptosis of human endometrial carcinoma cells

目的探讨α-氰基-N-苄苯乙烯胺(AG490)对人子宫内膜癌细胞系Ishikawa增殖和凋亡的影响。方法血清饥饿处理的Ishikawa细胞分别加入不同浓度的AG490(0、25、50、100μmol/L),作用不同的时间(24、48、72h)。噻唑蓝法检测各组细胞的增殖情况;平板克隆形成实验检测细胞克隆形成率;Hoechst33258免疫荧光观察细胞凋亡形态学特征;流式细胞术检测细胞周期和早期凋亡。结果AG490能明显抑制Ishikawa细胞的增殖,呈时间和剂量依赖性;并能显著地降低其单个细胞克隆形成率;AG490能将Ishikawa细胞阻滞于S期,并且诱导其凋亡,呈现时间和剂量依赖性。结论AG490可能成为治疗子宫内膜癌的新药物。

Objective To explore the effect of AG490 on the proliferation and apoptosis of human endometrial carcinoma cell line Ishikawa. Methods The cells after serum starvation were treated with AG490 with various concentrations （0, 25, 50, 100 μmol/L） for different durations （24, 48, 72 h）. The effect of AG490 on cell proliferation was examined by MTT assay; the cell cloning efficiency was measured by plate clone formation assay; the morphology of the cells was observed by using immunofluorescence （IF） of Hoechst 33258 staining; the cell cycle and early apoptosis were detected by flow cytometry （FCM）. Results AG490 inhibited the proliferation of Ishikawa cells in a time- and dose-dependent manner. AG490 significantly reduced the cloning efficiency of Ishikawa cells. The cell cycle was arrested in S phase by AG490 and AG490-induced apoptosis of Ishikawa cells in a time- and dose- dependent manner. Conclusion AG490 may be a new kind of treatment strategy for endometrial carcinoma.